Here are the 49 US AI startups that have raised $100M or more in 2025

2025: A Landmark Year for US AI Startups

2025 turned out to be a defining year for U.S. artificial intelligence startups. According to recent reports, 49 American AI companies secured funding rounds of US$100 million or more.

That total matches the previous year, proving that 2024 was not a fluke. The massive influx of capital into AI continues unabated.

The funding spans the full spectrum of AI applications, from infrastructure and compute to healthcare, enterprise software, legal tech, and voice and multimodal AI, showing investors’ broad confidence in the technology’s long-term potential.

What Kinds of AI Startups Got the Money

Infrastructure and Compute

The growth in AI infrastructure stands out as a major trend. For example, Cerebras Systems raised a US$1.1 billion Series G round, valuing it at US$8.1 billion.
Lambda, a startup providing AI cloud infrastructure, secured US$480 million in February, at a valuation of around US$2.5 billion.
Others like Groq pulled in US$750 million in a Series E round, signaling high demand for hardware and infrastructure optimized for AI workloads.

Healthcare, Legal and Enterprise AI

Startups applying AI to medical, legal, and business processes also did extremely well. Hippocratic AI raised US$126 million in Series C funding, reaching a valuation of US$3.5 billion.
Enterprise-focused companies offering AI search, automation, or workplace tools received significant backing, reflecting growing demand for AI-enhanced productivity.

Novel and Ambitious Ventures

Some funding rounds were truly massive even by startup standards. Reflection AI reportedly raised US$2 billion in Series B, a clear sign that investors are willing to double down on companies they believe can become future category-defining players.
The diversity in business models, from open-source model platforms, generative AI, agent frameworks, to specialized AI for voice, medical, or legal sectors, shows that the AI boom is not just about large language models or chatbots. It is far broader.

What This Means for the AI Industry

The wave of funding in 2025 suggests that the U.S. AI ecosystem may be entering a period of consolidation and maturation.

Strong investor interest in infrastructure providers could accelerate development of scalable, enterprise-ready AI tools. That would benefit companies and eventually consumers in every sector.
Big funding in healthcare and enterprise AI implies real-world applications are growing beyond hype. AI is getting serious about handling complex problems in medicine, business, and other professional fields.
The enormous capital flowing into early-stage and ambitious startups, even those not yet profitable, shows that investors expect long-term gains. Many of these companies might be aiming to build foundational platforms or future AI-native enterprises.

Ultimately, 2025’s funding story suggests that AI is no longer just about prototypes, hype, or experimentation. It is increasingly about building lasting infrastructure, tools, and services that can reshape industries.

What to Watch Next

As these companies scale, key developments to observe include:

Which startups successfully turn their funding into real products and market traction, especially in healthcare, enterprise, and infrastructure.
Whether consolidation begins, with big players acquiring smaller ones, or startups merging to combine strengths.
How regulatory, ethical, and societal challenges evolve with broad AI adoption, especially in sectors like healthcare and legal tech where risks and responsibilities are high.
The emergence of AI-driven tools and services accessible beyond the tech elite, showing how these investments might eventually impact everyday users and global markets.

The 2025 funding surge is a powerful signal. AI is moving from experimental labs to real-world systems, and the companies behind that shift are now backed by billions.